esp3i digested pxpr 502 Search Results


98
New England Biolabs esp3i
Esp3i, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 98 stars, based on 1 article reviews
esp3i - by Bioz Stars, 2026-03
98/100 stars
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94
Addgene inc esp3i digested pxpr 502

Esp3i Digested Pxpr 502, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 94 stars, based on 1 article reviews
esp3i digested pxpr 502 - by Bioz Stars, 2026-03
94/100 stars
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86
Thermo Fisher esp3i

Esp3i, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 86 stars, based on 1 article reviews
esp3i - by Bioz Stars, 2026-03
86/100 stars
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90
Addgene inc pxpr-502

Pxpr 502, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pxpr-502/product/Addgene inc
Average 90 stars, based on 1 article reviews
pxpr-502 - by Bioz Stars, 2026-03
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Image Search Results


Journal: iScience

Article Title: Genome-wide CRISPR-Cas9 screen analyzed by SLIDER identifies network of repressor complexes that regulate TRIM24

doi: 10.1016/j.isci.2023.107126

Figure Lengend Snippet:

Article Snippet: Oligonucleotides were resuspended to 100uM, annealed and treated with T4 PNK (NEB) at 10uM in 10uL reactions, diluted 1:200, and ligated into Esp3I (Thermo) digested lentiCRISPRv2 (Addgene 52961) for viral CRISPR-knockout by Cas9 or Esp3I digested pXPR_502 (Addgene 96923) for viral CRISPR-activation by dCas9-VP64 using 1uL diluted oligo and 100-200ng digested vector in 20uL T4 ligase (NEB) reactions.

Techniques: Virus, Recombinant, Mutagenesis, Protease Inhibitor, Membrane, SYBR Green Assay, Plasmid Preparation, cDNA Synthesis, Cell Culture, Purification, TA Cloning, Bicinchoninic Acid Protein Assay, Software